Propofol protects against oxidative-stress-induced COS-7 cell apoptosis by inducing autophagy
윤지영, 백철우, 김은정, 박봉수, 유수빈, 윤지욱, Kim Eok-Nyun,
소속 상세정보
윤지영 ( Yoon Ji-Young ) - Pusan National University School of Dentistry Department of Dental Anesthesia and Pain Medicine
백철우 ( Baek Chul-Woo ) - Pusan National University School of Dentistry Department of Dental Anesthesia and Pain Medicine
김은정 ( Kim Eun-Jung ) - Pusan National University School of Dentistry Department of Dental Anesthesia and Pain Medicine
박봉수 ( Park Bong-Soo ) - Pusan National University School of Dentistry Department of Oral Anatomy
유수빈 ( Yu Su-Bin ) - Pusan National University School of Dentistry Department of Oral Anatomy
윤지욱 ( Yoon Ji-Uk ) - Pusan National University School of Medicine Department of Anesthesiology and Pain Medicine
( Kim Eok-Nyun ) - Pusan National University School of Dentistry Department of Dental Anesthesia and Pain Medicine
KMID : 0980320170170010037
Abstract
Background: In oxidative stress, reactive oxygen species (ROS) production contributes to cellular dysfunction and initiates the apoptotic cascade. Autophagy is considered the mechanism that decreases ROS concentration and oxidative damage. Propofol shows antioxidant properties, but the mechanisms underlying the effect of propofol preconditioning (PPC) on oxidative injury remain unclear. Therefore, we investigated whether PPC protects against cell damage from hydrogen peroxide (H2O2)-induced oxidative stress and influences cellular autophagy.
Method: COS-7 cells were randomly divided into the following groups: control, cells were incubated in normoxia (5% CO2, 21% O2, and 74% N2) for 24 h without propofol; H2O2, cells were exposed to H2O2 (400 μM) for 2 h; PPC + H2O2, cells pretreated with propofol were exposed to H2O2; and 3-methyladenine (3-MA) + PPC + H2O2, cells pretreated with 3-MA (1 mM) for 1 h and propofol were exposed to H2O2. Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide thiazolyl blue (MTT) reduction. Apoptosis was determined using Hoechst 33342 staining and fluorescence microscopy. The relationship between PPC and autophagy was detected using western blot analysis.
Results: Cell viability decreased more significantly in the H2O2 group than in the control group, but it was improved by PPC (100 μM). Pretreatment with propofol effectively decreased H2O2-induced COS-7 cell apoptosis. However, pretreatment with 3-MA inhibited the protective effect of propofol during apoptosis. Western blot analysis showed that the level of autophagy-related proteins was higher in the PPC + H2O2 group than that in the H2O2 group.
Conclusion: PPC has a protective effect on H2O2-induced COS-7 cell apoptosis, which is mediated by autophagy activation.
키워드
Autophagy; COS-7 Cells; Oxidative Stress; Propofol
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